Human ELISA Kits

Human PTX3/Pentraxin 3 ELISA Kit PicoKine®

A sensitive ELISA assay for quantitative detection of human PTX3 in cell culture supernatants, serum, EDTA plasma and saliva.

CAT: 0519-EK0861   |   Size: 96 Well With Removable Strips

Product Overview

The Human PTX3/Pentraxin 3 ELISA Kit PicoKine® is designed for the quantitative measurement of human Pentraxin 3 (PTX3) in biological samples, including cell culture supernatants, serum, EDTA plasma and saliva.

PTX3 is a member of the pentraxin superfamily and plays an important role in innate immunity, pathogen recognition and inflammatory responses. It contributes to complement activation and supports the recognition of pathogens by macrophages and dendritic cells.

This ELISA kit provides sensitive and reproducible quantification of natural and recombinant human PTX3, making it suitable for research involving immune responses, inflammation and related biological processes.

Key Features & Benefits

• Quantitative detection of human PTX3/Pentraxin 3.
• Suitable for serum, EDTA plasma, saliva and cell culture supernatants.
• 96-well microplate with removable strips.
• Detection range: 312 pg/mL – 20,000 pg/mL.
• High sensitivity: less than 10 pg/mL.
• Designed for reproducible and precise measurements.
• No detectable cross-reactivity with other relevant proteins.
• Recognizes natural and recombinant human PTX3.

Biological Background

PTX3, also known as Pentraxin-related protein PTX3 or TNF alpha-induced protein 5 (TNFAIP5), belongs to the long pentraxin family. The PTX3 gene is located on chromosome 3q25.32 and encodes a secreted protein involved in innate immune defense.

PTX3 participates in the regulation of resistance to pathogens, inflammatory reactions and the classical complement pathway. It also facilitates pathogen recognition by immune cells.

Synonyms: Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, TNF alpha-induced protein 5, TSG-14, PTX3, TNFAIP5, TSG14.

Applications & Research Areas

• ELISA-based protein quantification.
• Innate immunity research.
• Inflammation and immune response studies.
• Pathogen recognition research.
• Neuroscience research.
• Transcription-related research.

Technical Specifications

Product: Human PTX3/Pentraxin 3 ELISA Kit PicoKine®

Catalog Number: 0519-EK0861

Size: 96 Tests

Plate Format: Pre-coated 96-well strip microplate

Reactivity: Human

Gene Name: PTX3

Gene ID: 5806

UniProt: P26022

Immunogen: E18–S381

Expression System for Standard: NS0

Capture Antibody: Goat polyclonal antibody

Detection Antibody: Goat polyclonal antibody

Standard: 20 ng/Tube

Detection Range: 312 pg/mL – 20,000 pg/mL

Sensitivity: Less than 10 pg/mL

Specificity: Natural and recombinant human PTX3

Anticoagulant: EDTA

Calculated Molecular Weight: 41,976 MW

Subcellular Location: Secreted

Application: ELISA

Dry Ice: No

Hazardous: No

Kit Components

The kit includes the following reagents and materials:

• Pre-coated 96-well strip microplate – 1
• Standard – 2 vials
• Biotinylated antibody (100x) – 100 µL
• Avidin-Biotin-Peroxidase Complex (100x) – 100 µL
• Sample Diluent – 30 mL
• Antibody Diluent – 12 mL
• Avidin-Biotin-Peroxidase Diluent – 12 mL
• Color Developing Reagent (TMB) – 10 mL
• Stop Solution – 10 mL
• Wash Buffer (25x) – 20 mL
• Adhesive plate sealers – 4

Assay Precision & Reproducibility

Intra-Assay Precision

Sample concentrations: 662, 1701 and 9194 pg/mL.

Coefficient of variation (CV): 6.9%, 5.0% and 6.9%, respectively.

Inter-Assay Precision

Sample concentrations: 718, 1816 and 9601 pg/mL.

Coefficient of variation (CV): 5.4%, 6.9% and 6.9%, respectively.

Lot-to-Lot Reproducibility

• Sample 1: Mean 644 pg/mL; CV 5.4%.
• Sample 2: Mean 1601 pg/mL; CV 3.9%.
• Sample 3: Mean 8974 pg/mL; CV 1.7%.

Storage Conditions

Store the kit at 4°C for up to 6 months or at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles.

The kit is shipped with gel ice and can be stored at room temperature for up to 3 days during transit. Freeze upon receipt according to the recommended storage conditions.

Materials Required But Not Provided

• Microplate reader capable of measuring absorbance at 450 nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips suitable for dispensing 0.5 µL to 1 mL volumes.
• Multichannel pipettes recommended for processing larger sample quantities.
• Deionized or distilled water.
• 500 mL graduated cylinders.
• Test tubes for dilution.

Order Human PTX3/Pentraxin 3 ELISA Kit

Discover reliable ELISA solutions for protein quantification and immunology research.

24/48H Stock Items & 2 to 6 Weeks non Stock Items.